Caffeine Lab Report - 769 Words

Isolation of a Natural product, in the experiment caffeine [C8H10N4O2], is based on a series of procedures. Firstly, it is important to examine the structure of caffeine which is an alkaloid. Typically alkaloids are nitrogen based and found in plants, caffeine itself is composed of a purine ring with functional groups of a amide, amine and alkene respectively. Furthermore, Caffeine includeds a lone pair on the nitrogen atom asserting several functional properties(Zhao et al. 206). Under examination of the caffeine structure several intermolecular forces are present including hyrdogen bonding, dipole dipole forces and Van der Waals forces. What this indicates is that caffeine is a polar molecule and reacts moderatley hydrophobically in the†¦show more content†¦In the solid-liquid extraction all water soluble components were sepreated along with the caffeine including the tannins. To further isolate the caffeine and obtain a purer product it was necessary to add sodium carbona te(Zhao et al. 208). This acidic- base reaction (tannins being the acid electrophile and sodium carbonate the base nucleophile) forms conjugate base phenolic salts. Tannins are acidic and thus have an–OH group directly bound to their aromatic ring the addition of a weaker base allows for the deprotonation of said –OH group. Then when the methylene chloride was added the tannins, now a phenolic salt, were insoluble thus resulting in the formation of two layers (organic and aqeous). The aqeuous layer now contains the tannins and chlorphyll and the organic layer the caffeine. This occurs because caffeine is more soluble in the methylene chloride and thus is separtaed into the same layer and the tannin salts and chlorophyll which are insoluble in the methylene chloride remain in the aqeous layer. The organic layer is extracted and undergeos this same washing process again in order to extract the maximum amount of product. Once enough caffeine was extracted a drying agen t of anyhydrous sulfate was added to remove any of the excess water in the organic layer and placed in a hot chamber to evaporate off the methylene chloride. The remaining crystals underwent recrystalization to obtain a pure substance. (Zhao et al. 208). Additional caffeine extractionShow MoreRelatedMelting Point Of Caffeine Lab Report1937 Words   |  8 Pagesisolation of a natural product from a biological source using extraction techniques, and to demonstrate purification by sublimation. We are isolating caffeine from tea. The goals of the experiment are to successfully extract caffeine from the tea solution and to further purify it by sublimation. We will find the percent yield and melting point of the caffeine at the end and we will also identify certain functional groups using an infrared spectroscopy and ‘H NMR. Main Reactions: Procedure: The experimentRead MoreEssay Separation of Benzoic Acid and Acetanilide1243 Words   |  5 PagesUnknown #: 146 Fall-11 Organic Chemistry Midterm Report 13 October 2011 Table of Contents Separation of Benzoic Acid and Acetanilide 3 Extraction and Purification of Caffeine from Tea 4 Extraction of Trimyristin from Nutmeg 5 Conversion of t-butanol to t-butyl chloride 6 Appendix 7 Calculations 8 Literature and Experimental Values of Benzoic Acid and Acetanilide 9 Experimental Caffeine IR 10 Literature Caffeine IR 11 Experimental Trimyristin IR 12 LiteratureRead MoreThe Effects Of Alcohol On The Heart Rate Of Daphnia Magna1341 Words   |  6 PagesABSTRACT This report presents the physiological changes in the heart rate of a fresh water crustacean Daphnia magna when exposed to caffeine and alcohol. Different Daphnia magnas were placed in a depression slide containing fresh water and later exposed to solutions of caffeine and alcohol. Each Daphnia magna had different responses. These data suggest there is an increase in heart rate when a Daphnia magna is exposed to caffeine and a decrease in heart rate when it is in a solution of alcohol. Read MoreNCAA Drug Testing707 Words   |  3 Pagesagents, which scored a 1.1% usagea at the college level,in the Study of Substance Use and Abuse habits(Green). Steroids are mostly taken by men, because they act as a testosterone. Caffeine, which is both a diuretic and a stimulant. caffeine is illegal in excessive amounts of 3-13 mg/kg of body weight(Maria). This much caffeine may improve ones results in endurance events (drug free sport) but can also cause harmful symptoms such as â€Å"Premature l eft ventricular contractions, and elevated heart rate andRead MoreAnalysis of Research on Caffeines Effects on Mood Dimensions 1585 Words   |  6 Pagesaddressing? The author is addressing whether caffeine can have an affect on mood dimensions of arousal versus a subject feeling pleasant. The author also addresses whether these arousals will influence memory in either of two ways. First, arousal through learning, and second through altered mood states through learning and recall (Herz,1999). B) What have other researchers already discovered about this topic? Other researchers have found that caffeine is one of the most psychoactive substances consumedRead MoreOrganic Chem 1 Post Lab Report Essay848 Words   |  4 PagesPost Lab Report Experiment 3 - Chromatography – Analyzing Analgesics by TLC and Isolation of ÃŽ ²Ã¢â‚¬â€œCarotene by Column Chromatography Chemicals 1. Acetaminophen (C8H9NO2) 2. Aspirin (C9H8O4) 3. Caffeine (C8H10N4O2) 4. Ibuprofen (C13hH18O2) Introduction In this experiment, several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions byRead MoreAdvantages And Disadvantages Of Western Medicine Essay1140 Words   |  5 Pageswould be that the diagnosis and treatment of epilepsy was not as advanced as today’s practices. Compare and Contrast: Nurse’s Role/Teaching Western Medicine Patients must also be taught to report bleeding due to blood dyscrasias, change in vision, wear sun protective clothing, avoid alcohol, nicotine, and caffeine, make position changes slowly because of the potential of orthostatic hypotension, maintain oral hygiene for the risk of gingival hyperplasia, use barrier method of contraception, and toRead MoreA Study On The History Of Coffee Essay1621 Words   |  7 Pagesplanted worldwide. Part II: The Significance of Coffee in Modern Life Every day, the modern world consumes 300 tons of caffeine, or one cup of coffee for every man, woman, and child. Coffee is the second most traded commodity on Earth, after oil. The world’s largest buyer of coffee is now the United States, which has to import nearly all of this as the coffee trees from which caffeine is harvested will only grow at commercial levels between the Tropic of Cancer and the Tropic of Capricorn, otherwiseRead MoreExperiment 2a Adsorption Chromatography ( Tlc )1455 Words   |  6 PagesItem Possible Points Actual Points Pre-Lab 2 Notebook: N/A N/A Purpose/Table of Reagents 2 Corrections 2 Blank Spaces 2 Signatures 2 TLC data (4-in notebook) 8 Coherent 2 Conclusions (absent here) 1 Sub-Total = 21 multiply Sub-Total x 2= 42 Report: N/A N/A Introduction 2 Data and Calculations 8 Less Points-Missing Data N/A N/A Unknown Identity 10 Data Analysis / Conclusions 5 TOTAL 67 minus any page overage 0 minus for late reports 0 minus for TA points 0 FINAL TOTALRead MoreBirth Of A Diabetic Mother1530 Words   |  7 Pagesage of 35, women with a history of spontaneous abortions, cigarette smoking, ETOH use, recreational drug use, high doses of caffeine, uncontrolled maternal diabetes mellitus (DM), uncontrolled maternal hypertension (HTN) Signs and Symptoms Abdominal or suprapubic pain, bleeding, expulsion of tissue Late abortions may begin with gush of fluid Massive hemorrhage is rare Labs Blood typing, CBC, HCG, WBC Treatment Observation (if abortion is threatening) DC Medications to dilate uterus or expel fetus